Osteochondral Graft Size Is Significantly Associated With Increased Force and Decreased Chondrocyte Viability
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چکیده
منابع مشابه
Chondrocyte viability in press-fit cryopreserved osteochondral allografts.
The viability of chondrocytes in press-fit glycerol-preserved osteochondral allografts was compared to that in fresh autografts, after transplantation into load-bearing and non-load-bearing sites in mature sheep stifle joints. We used macroscopic grading, tonometer pen indentation testing, histology, sulfate uptake and viability as determined by confocal-microscopy to assess cartilage condition...
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This open-access article is published and distributed under the Creative Commons Attribution NonCommercial No Derivatives License (http://creativecommons.org/licenses/by-nc-nd/3.0/), which permits the noncommercial use, distribution, and reproduction of the article in any medium, provided the original author and source are credited. You may not alter, transform, or build upon this article witho...
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متن کاملChondrocyte viability in fresh and frozen large human osteochondral allografts: effect of cryoprotective agents.
Chondrocyte survival is a major goal for the effective storage and clinical performance of human osteochondral allografts. The majority of animal and human cryopreservation studies conducted so far have been performed in small osteochondral cylinders. Using human tibial plateaus as a model for large osteochondral pieces, this work sought to evaluate the cryoprotective efficiency of glycerol and...
متن کاملProlonged storage of osteochondral allografts: does the addition of fetal bovine serum improve chondrocyte viability?
Osteochondral plugs were harvested from eight fresh human femoral condyles within 96 hours of donor death. The plugs were either stored in a serum-free media containing glucose, salts, and amino acids or 10% fetal bovine serum at 4 degrees C. After 28 days of storage, the osteochondral plugs were analyzed for chondrocyte viability and viable cell density using confocal microscopy, proteoglycan ...
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ژورنال
عنوان ژورنال: The American Journal of Sports Medicine
سال: 2018
ISSN: 0363-5465,1552-3365
DOI: 10.1177/0363546517748906